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Primo at Wiki-it! | You need to have an account in order to use this site. Registration is free!Fabrication and characterization of nanoparticles with poly(lactic-co-glycolic acid) for delivery of 2,4-dichlorophenoxyacetic acid into the brain.
We have previously reported the synthesis of PLGA nanoparticles to deliver agricultural chemicals into the brain using our recently developed olfactory-triggered brain-stimulating nanodevice (PTBND). However, the potential hazard associated with the toxic agrichemical 2,4-dichlorophenoxyacetic acid (2,4-D) may limit the potential use of PTBND for agricultural applications. In this study, 2,4-D-loaded PLGA nanoparticles were prepared using an emulsion/solvent evaporation method followed by sodium cholate coating for improving the retention of nanoparticles in the brain. The size and size distribution of the 2,4-D-loaded PLGA nanoparticles were relatively uniform with an average size of 153.3 nm and a standard deviation of 19.1 nm. The stability of the nanoparticles was examined by measuring their size for up to 24 h after preparation. The uptake and distribution of 2,4-D in the brain were examined in rodents following the olfactory-triggered administration of 2,4-D-loaded PLGA nanoparticles. The uptake of 2,4-D was considerably lower in the brain when compared to that observed following the administration of a commercial foliar spray formulation. More importantly, there was no distribution of 2,4-D into the brain following the olfactory-triggered administration of PLGA nanoparticles. In summary, the PLGA nanoparticles prepared in this study do not appear to be suitable for the brain-specific delivery of 2,4-D.Trauma-related dissociative symptoms in a clinical sample of children and adolescents.
The main purpose of the present study was to assess the frequency of trauma-related dissociative symptoms in children and adolescents. An analysis was performed of self-reported trauma-related dissociative symptoms in a clinical sample of patients attending an out-patient trauma clinic for acute or post-traumatic stress disorder (PTSD ac619d1d87
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